Coding

Part:BBa_K3787002:Design

Designed by: Leung Gabriel, Tam Ching Yeung, Wong Chun Hei   Group: iGEM21_HK_GTC   (2021-09-28)


S245I PETase-MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
    Illegal NheI site found at 1038
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
    Illegal NotI site found at 1586
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
    Illegal NgoMIV site found at 1513
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

It is a chimeric protein.The part coding for both PETase and MHETase comes from the gene of the bacteria I. sakaiensis. Moreover, the part coding for PETase is modified.

References